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primary antibodies nfat4  (GeneTex)

 
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    GeneTex primary antibodies nfat4
    Primary Antibodies Nfat4, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies nfat4/product/GeneTex
    Average 90 stars, based on 1 article reviews
    primary antibodies nfat4 - by Bioz Stars, 2026-02
    90/100 stars

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    BTP2 inhibited the EPI-induced nuclear translocation of <t>NFAT4</t> in HL-1 cardiomyocytes. HL-1 cells were treated with vehicle, 1 µM EPI, 20 µM BTP2, or 1 µM EPI combined with 20 µM BTP2 for 5 h, followed by drug withdrawal and culture for 24 h and stained for NFAT4. Cells treated with 10 µM ionomycin for 15 min were used as positive control. ( A ) Representative confocal images of NFAT. Scale bar, 10 µm. white arrows indicate the co-localization of NFAT4 and Nuclei ( B ) Statistical analysis of the percentage of cells with NFAT nuclear translocation. Each dot is an averaged datapoint from a single confocal image. Each image represents one biological replicate. Mean ± SD. **: p < 0.01 (based on one-way ANOVA and Bonferroni post hoc analysis).
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    BTP2 inhibited the EPI-induced nuclear translocation of NFAT4 in HL-1 cardiomyocytes. HL-1 cells were treated with vehicle, 1 µM EPI, 20 µM BTP2, or 1 µM EPI combined with 20 µM BTP2 for 5 h, followed by drug withdrawal and culture for 24 h and stained for NFAT4. Cells treated with 10 µM ionomycin for 15 min were used as positive control. ( A ) Representative confocal images of NFAT. Scale bar, 10 µm. white arrows indicate the co-localization of NFAT4 and Nuclei ( B ) Statistical analysis of the percentage of cells with NFAT nuclear translocation. Each dot is an averaged datapoint from a single confocal image. Each image represents one biological replicate. Mean ± SD. **: p < 0.01 (based on one-way ANOVA and Bonferroni post hoc analysis).

    Journal: Cells

    Article Title: Blocking Store-Operated Ca 2+ Entry to Protect HL-1 Cardiomyocytes from Epirubicin-Induced Cardiotoxicity

    doi: 10.3390/cells12050723

    Figure Lengend Snippet: BTP2 inhibited the EPI-induced nuclear translocation of NFAT4 in HL-1 cardiomyocytes. HL-1 cells were treated with vehicle, 1 µM EPI, 20 µM BTP2, or 1 µM EPI combined with 20 µM BTP2 for 5 h, followed by drug withdrawal and culture for 24 h and stained for NFAT4. Cells treated with 10 µM ionomycin for 15 min were used as positive control. ( A ) Representative confocal images of NFAT. Scale bar, 10 µm. white arrows indicate the co-localization of NFAT4 and Nuclei ( B ) Statistical analysis of the percentage of cells with NFAT nuclear translocation. Each dot is an averaged datapoint from a single confocal image. Each image represents one biological replicate. Mean ± SD. **: p < 0.01 (based on one-way ANOVA and Bonferroni post hoc analysis).

    Article Snippet: Then, the cells were incubated with rabbit anti-NFAT4 primary antibody (1:100, ProteinTech, Rosemont, IL, USA) in blocking solution at 4 °C overnight.

    Techniques: Translocation Assay, Staining, Positive Control

    Working model. Acute treatment of EPI enhanced SOCE, triggering NFAT4-mediated hypertrophy and caspase3-mediated apoptosis in cardiomyocytes. Both the EPI-induced apoptosis and hypertrophy can be inhibited by blocking SOCE.

    Journal: Cells

    Article Title: Blocking Store-Operated Ca 2+ Entry to Protect HL-1 Cardiomyocytes from Epirubicin-Induced Cardiotoxicity

    doi: 10.3390/cells12050723

    Figure Lengend Snippet: Working model. Acute treatment of EPI enhanced SOCE, triggering NFAT4-mediated hypertrophy and caspase3-mediated apoptosis in cardiomyocytes. Both the EPI-induced apoptosis and hypertrophy can be inhibited by blocking SOCE.

    Article Snippet: Then, the cells were incubated with rabbit anti-NFAT4 primary antibody (1:100, ProteinTech, Rosemont, IL, USA) in blocking solution at 4 °C overnight.

    Techniques: Blocking Assay